Sister Act is a 1992 American comedy film released by Touchstone Pictures. Directed by Emile Ardolino, it features musical arrangements by Marc Shaiman and stars Whoopi Goldberg as a Reno lounge singer who has been put under protective custody in a San Francisco convent and has to pretend to be a nun when a mob boss puts her on his hit list. Also in the cast are Maggie Smith, Kathy Najimy, Wendy Makkena, Mary Wickes, and Harvey Keitel. The film is #83 on Bravo's The 100 Funniest Movies list.

The film was followed by a 1993 sequel, Sister Act 2: Back in the Habit. It also inspired a musical stage version that premiered at the Pasadena Playhouse in Pasadena, California in 2006.